Journal: The Journal of Biological Chemistry

Article Title: Urokinase-type plasminogen activator (uPA) is critical for progression of tuberous sclerosis complex 2 (TSC2)-deficient tumors

doi: 10.1074/jbc.M117.799593

Figure Lengend Snippet: Up-regulation of uPA in human LAM and angiomyolipoma tissue samples. A, normal human lung; B, human LAM nodules; C, normal human kidney; and D, renal angiomyolipoma. uPA was detected using a mouse anti-uPA monoclonal antibody (mAb) followed by Vectastain ABC combined with the Alexa Fluor 488-tyramide amplification system (green). SM α-actin was detected using Cy3-conjugated mouse SM α-actin mAb (red). Nuclei were counterstained with DAPI (blue) (upper panels). Total mouse IgG served as the negative controls for uPA staining, and Cy3-conjugated mouse anti-SM α-actin mAb was used to locate the same tumor nodules in serial sections (bottom panels). Additional serial sections were incubated with Cy3-conjugated total mouse IgG as the negative control for SM α-actin staining (data not shown). Images were taken using an LSM710 microscope. Scale bar, 50 μm. Data shown are representative of six LAM lesions from four patients and four angiomyolipomas from individual patients.

Article Snippet: Sections were incubated overnight at 4 °C with primary mouse monoclonal anti-human uPA antibody (American Diagnostica, catalog no. 3689) diluted in blocking buffer.

Techniques: Amplification, Staining, Incubation, Negative Control, Microscopy